Cell Culture Protocols

Wheeler Lab Basic Cell Culture Protocols can be found in PDF format on this page or as separate wiki-pages.

See "Culture of Animal Cells" by R. Ian Freshney for supplementary protocols and cell culture reading.


  1. Passaging - Adherent Cells
  2. Passaging - Suspension Cells
  3. Thawing
  4. Cryopreservation
  5. Expansion

Note : for devices that are going to be put in the incubator, you need to seal the Parylene edges to prevent water from getting in. Sam tested a bunch of different tapes, glues, etc. and settled on this one from McMaster-Carr.