Safety Manual

General Resources

1. Safety Data Sheet Lookup
2. Lab Manual
3. UofT Biosafety Manual
4. Canadian Biosafety Standards 2nd Ed.
5. Handling and Disposing of Sharps
6. Chemical Spills (on the body)
7. Biological Spills
8. Biological Disposal (Waste Information and Procedures for Bio Labs)
9. Biological Materials Inventory
10. Other Emergencies (fire, medical, etc.)
11. Evacuation and Contacts
12. Faculty of Medicine 2019 Update to Chemical Waste Labelling
13. Intro to CCBR Waste Disposal

MSDS and Chemical SOPs

1. Chemwatch
2. Chemwatch User Guide
3. UofT Hazardous Materials Information
4. Printing Workplace Labels for Chemicals (and template files)
5. Liquid Nitrogen Handling SOP

Lash Miller Health and Safety SOPs*

1. Emergency Response
2. Crisis Response
3. Crisis Response Guide
4. Chemical Compatibility and Storage
5. Chemical Spills
6. Chemical Waste Management
7. Fume Hood Use
8. Cryogen Dispensing
9. Cryogen Dewar Change
10. Transporting Cryogenic Liquids
11. First Aid
12. Chemical Inventory Management
13. Emergency Purge Button Procedure

Risk Assessments

1. Risk Assessment Measles
2. Risk Assessment Rubella
3. Risk Assessment HUVEC (SDS)
4. Risk Assessment Human Blood (SDS)
5. Risk Assessment Rubella Antigen
6. Risk Assessment Measles Antigen
7. Risk Assessment HeLa (SDS)
8. Risk Assessment BaF3 (SDS)
9. Risk Assessment for FFPE human prostate biopsies
10. Risk Assessment for human endometrial tissue samples
11. Risk Assessment for laboratory-bred mouse and rat tissue
12. Risk Assessment Human urine (SDS Synthetic Urine)
13. Risk Assessment Paramecium
14. Risk Assessment Sheep Blood(SDS)
15. Risk Assessment retinal pigmented epithelium cells
16. Risk Assessment C elegans
17. Risk Assessment SH-SY5Y
18. Risk Assessment PC12 (SDS)
19. Risk Assessment NSC
20. Risk Assessment MDCK (SDS)
21. Risk Assessment CHO-K1 (SDS)
22. Risk Assessment Human Sperm
23. Risk Assessment HepG2 (SDS)
24. Risk Assessment Mcf-7 (SDS)
25. Risk Assessment MDA-MB-231 (SDS)
26. Risk Assessment NIH-3T3 (SDS)
27. Risk Assessment Primary trophoblast cells
28. Risk Assessment U87 MG (SDS)
29. Risk Assessment B-16 (SDS)
30. Risk Assessment CHO-DG44 (SDS)
31. Risk Assessment H1 (SDS)
32. Risk Assessment SARS-CoV-2
33. Risk Assessment HL-60 (SDS)
34. Risk Assessment MEF
35. Risk Assessment HEK293T
36. Risk Assessment BV-2

Equipment SOPs

1. DropBot SOP
2. Soldering SOP
3. Laser Cutter SOP
4. Agilent API SOP
5. UV Lamp SOP
6. Spin Coater SOP
7. Oven SOP
8. Gas Cylinders SOP
9. Glass cutter SOP
10. Biosafety Cabinet SOP
11. Eksigent nanoLC and LTQ SOP
12. Form 2 Resin Printer SOP
13. Ultra 50 Laser SOP
14. Ultimaker 3d Printer SOP
15. Roll Coater SOP
16. Thermal Cycler SOP
17. LM Fume Hood SOP
18. Pressure Injection Cell SOP
19. Centrifuge SOP (AccuSpin Micro 17/17R / Eppendorf Microcentrifuge 5430)
20. HarvestRight Freeze Dryer SOP

Biological SOPs

1. Cell Culture Protocols
2. Bacteria Protocols
3. Blood Collection
4. Handling of human samples for diagnostic assays on digital microfluidics
5. Storage and handling of human samples suspected of measles or rubella infection
6. Virus coated magnetic microparticles
7. Handling and storage of pooled human saliva
8. Handling and storage of human serum samples for measles and rubella IgG and IgM
9. Handling of human urine for diagnostic assays on digital microfluidics
10. Sheep Blood
11. Retinal pigmented epithelium cells
12. Paramecium
13. C elegans
14. Working with human or animal tissue samples on digital microfluidics
15. Biological Fluid Sample Transport
16. Pacific Hemostasis Fibrinogen Degradation Products (FDP) Assay Kit
17. Antigens For typing of AB0 blood
18. Anti-D (RH1) Blend
19. Safety Procedures with Biologicals in Biosafety Cabinets
20. Standard Operating Procedure, Buccal sample collection, storage and use
21. Standard Operating Procedure: SARS-CoV-2

Equipment Maintenance Logs

* These SOPs are all required reading for key access to LM. They can all be dowloaded together here.